We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus, a model for developmental and systems biology. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome… Analysis of the genome of Fugu rubripes (pufferfish) revealed that this duplication was even more ancient. … purpuratus was chosen for a number of reasons, including the detailed understanding of many molecular mechanisms underpinning early development and the availability of resources such as arrayed cDNA … It has been shown that although the Fugu rubripes genome is only one-eighth the size of the human genome, it contains a comparable complement of protein-encoding genes . The S. purpuratus was sequenced using the Clone-Array Pooled Shotgun Sequencing (CAPSS) method where shotgun libraries are made from row and column pools of arrayed BACs from an FPC generated tiling path (BC Genome Sciences Center).The deconvoluted individual BAC sequences as well as assemblies enriched with WGS sequences from the BAC region are available in GenBank. Strongylocentrotus purpuratus: Developmental transcriptomes of the purple sea urchin was obtained from SRA (Accession: PRJNA81157), sequenced by … We found five sequences on different scaffolds in the pufferfish genome that encode predicted proteins containing a … Components i. Download View all proteins. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. Proteomes - Strongylocentrotus purpuratus (Purple sea urchin) ))) All None.
GENOME: Strongylocentrotus purpuratus (purple sea urchin) Entry: T01019 Complete Genome : Name: spu, STRPU, 7668. The recent publication of the Strongylocentrotus purpuratus genome sequence rendered possible not only the identification of possible matrix proteins at the gene level, but also the direct identification of proteins contained in matrices of skeletal elements by in-depth, high-accuracy, proteomic analysis. All of the sequence data that we maintained at SUGP was incorporated into the new web site while preserving the extensive primary annotations made in conjunction with the primary sequencing of the purple sea urchin (Strongylocentrotus purpuratus) genome. The reference proteome is derived from the genome sequence published in 2006. The first echinoderm genome sequenced and assembled was that of the purple sea urchin (Strongylocentrotus purpuratus) (Sea Urchin Genome Sequencing Consortium et al., 2006).S. Of course, these data are also available in public sequence databases such as the National Center for Biological Information. The purple sea urchin genome has 21 pairs of autosomes and one pair of sex chromosomes containing 814 Mb with 23,000 protein-coding genes.
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