Lazzaro Spallanzani was an Italian physiologist who extensively studied animal biology and reproduction.He is probably most famous for his experiments that helped to disprove the theory of spontaneous generation, which helped to pave the way for future research by Louis Pasteur (1822-1895). Italian Physiologist. Spallanzani Lazzaro Spallanzani, Italian physiologist who made important contributions to the experimental study of bodily functions and animal reproduction. One flask was left opened, one flask was sealed, one flask was … Spallanzani proposed that the microorganisms could have entered the flask after it was boiled, and before it was sealed. In order to test Needham's theory, Spallanzani put broth in a flask, sealed the … In Lazzaro Spallanzani's Experiment he proved microorganisms could be killed by boiling. [3] As in Needham’s experiment, broth in sealed jars and unsealed jars was infused with plant and animal matter. However, when Spallanzani heard of this experiment, he did not believe it. Lazzaro Spallanzani (1729–1799) did not agree with Needham’s conclusions, however, and performed hundreds of carefully executed experiments using heated broth. His investigations into the development of microscopic life in nutrient culture solutions paved the way for the research of Louis Pasteur. 1729-1799. At the half of the 18th century a young Italian abbot, Lazzaro Spallanzani, Professor of Physics and Mathematics at the University of Reggio Emilia, started repeating the experiments of John Turberville Needham. Lazzaro Spallanzani. He believed microbes move through the air and could be killed by boiling. Spallanzani filled 4 flasks with a broth.