240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected] FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. Lambda DNA digested with BsmBI (Esp3I), 1% agarose, 14 cleavage sites.
Brand: Thermo Scientific™ FD0454 Code : 88. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Thermo Scientific Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer. 5'CGTCTCN13'3'GCAGAGN55'Thermo Scientific FastDigest Esp3I (IIs class) restriction enzyme recognizes CGTCTC(1/5)^ site and cuts best at 37C in 515 minutes using universal FastDigest Buffer.
Note: Also available as a FastDigest enzyme for rapid DNA digestion. Citations: Isoschizomers: BsmBI, BstGZ53I, Esp16I, Esp23I.Thermo Scientific FastDigest Esp3I is …
Description. Description The FastDigest BsmBI (Esp3I) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal Yellow Tango Buffer Be the first to write a review! Together with the polymerase chain reaction (PCR), restriction enzymes (REs) are arguably the most important tools in molecular biology. FastDigest BsmBI (Esp3I) (20 rxns) from Thermo Fisher Scientific. BsmBI compatible tails, 5’CACCGX3’ and 5’AAACYC3’, with X and Y being complementary sequences to the sgRNA, were added to each oligonucleotide.
Restriction enzymes continue to be important In 2006, the innovative Lithuanian biotech company Fermentas launched a new product line for molecular biology researchers: the FastDigest restriction enzymes. plentiCRISPRv2 vector was digested with FastDigest BsmBI (Fermentas) for 30 min at 37°C and gel-purified using a 0.8% agarose gel.